Interactions between newcastle disease virus (NDV), antibody and cell

摘要: Abstract The present work is a quantitative study of the inactivation by antibody two measurable properties Newcastle disease virus—infectivity and enzymatic activity—and role played cells in this inactivation. By use P32-labeled virus, enzyme, measured capacity virus to elute from red blood (RBC), shown be inactivated according first order kinetics. Repetition adsorption-elution cycles shows that there are multiple enzyme sites on virus. A method introduced for determining number per particle. fraction which remains infective after treatment largely retains its infectivity when firmly adsorbed cells. On other hand fully untreated loses upon firm adsorption. This observation leads conclusion more than one site particle at can occur, as text. None critical sense no single determines, priori, inactive. Calculation these data “infectivity” agrees magnitude with calculated was derived an independent technique. Evidence presented reactivation virus-antibody complexes surface cell. blocked if serum medium. relevance findings various theories inactivation, cell penetration discussed.