p73 expression for human buccal epithelial dysplasia and squamous cell carcinoma: does it correlate with nodal status of carcinoma and is there a relationship with malignant change of epithelial dysplasia?

摘要: Background. TP73, a p53 homologue gene, shares similar structural sequences with p53. The aim of this study was to investigate the p73 expression for human buccal epithelial dysplasia (ED) and squamous cell carcinoma (SCC). Methods. Seventy-five samples ED, including mild, moderate, severe ED (25 in each category), were analyzed protein by means immunohistochemistry. Twenty-five SCCs mRNA reverse transcription-polymerase chain reaction (RT-PCR) also immunohistochemical analysis. Results. By use analysis, nuclear staining detected subset normal mucosa, SCC specimens. noted basal cells mucosa. For lesions deriving from observed parabasal layers more superficial corresponding spinous layer. well-differentiated carcinomas, immunoreactivity chiefly among less-differentiated periphery carcinomatous clusters, whereas moderately differentiated carcinomas revealed homogeneous staining, involving nearly all tumor cells. On RT-PCR, be compatible findings analysis. An electrophoretic band 180-bp PCR product has been observed. seemed significantly elevated specimens (protein level) levels) compared analogous control tissue (Fisher's exact test, p .05). Conclusions. The data suggest that may (1) associated differentiation oral stratified epithelium, (2) an early event carcinogenesis, (3) nodal status patients possible indicator malignant change ED. © 2004 Wiley Periodicals, Inc. Head Neck26: 945–952,