In vitro effects of ethanol withdrawal and spermidine on viability of hippocampus from male and female rat.

摘要: Background: Long-term ethanol dependence results in neuronal adaptation that likely contributes to withdrawal-induced central nervous system excitability and, potentially, neurotoxicity. This has been suggested result, part, from increased release of or response endogenous polyamines. Furthermore, it reported neurological difficulties related and withdrawal may be more severe female than male alcoholics. Thus, we designed this study examine effects the polyamine spermidine on neurotoxicity associated with long-term exposure by using organotypic hippocampal slice cultures derived rats. Methods Results: Twenty-four hours after continuous 10 day (100 mM culture medium) resulted cytotoxicity explants obtained both sexes. was most evident pyramidal cell layers CA1 region, no sex differences were observed severity damage. Exposure sexes NMDA blocker MK-801 during significantly reduced toxicity. In control cultures, μM) alone produced significant similar rats. all regions explants. CA3 spermidine-potentiation damage greater rats compared those rats. Conclusions: These data demonstrate presence is mediated, overactivation receptors. these findings suggest females susceptible males polyamine-mediated exposure.