Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation

摘要: Cryoprotectants (CPAs) play a critical role in cryopreservation because they can resist the cell damage caused by freezing process. Current state-of-the-art CPAs are mainly based on an organic solvent dimethyl sulfoxide (DMSO), and several DMSO-cryopreserved products have been brought to market. However, intrinsic toxicity complex protocol of DMSO still remain as bottleneck wide use for clinical applications. Herein, we reported that betaine, natural zwitterionic molecule, could serve nontoxic high efficient CPA. At optimum concentration different types exhibited exceptional post-thaw survival efficiency with ultrarapid protocol, which was straightforward, cost but difficult succeed using DMSO. Moreover, betaine showed negligible cytotoxicity even after long-term exposure cells. Mechanistically, hypothesized be ultra-rapidly taken up cells intracellular protection during This technology unlocks possibility alternating traditional toxic is applicable variety