Characterization of Antigen-Specific Immune Responses Induced by Canarypox Virus Vaccines

摘要: Avipoxvirus-based vectors, such as recombinant canarypox virus ALVAC, are studied extensively delivery vehicles for vaccines against cancer and infectious diseases. Effective use of is expected to benefit from proper understanding the interaction between these viral vectors host immune system. We performed preclinical vaccination experiments in a murine tumor model analyze immunogenic properties an ALVAC-based vaccine carcinoembryonic Ag (ALVAC-CEA), tumor-associated autoantigen commonly overexpressed colorectal cancers. The protective CEA-specific immunity induced by this consisted CD4 + T cell responses with mixed Th1/Th2 cytokine profile that were accompanied potent humoral responses, but not CD8 CTL immunity. In contrast, DNA (DNA-CEA) Th1 responses. Modification ALVAC-CEA through coinjection DNA-CEA, admixture CpG oligodeoxynucleotides, or supplementation additional transgenes encoding triad costimulatory molecules (TRICOM) did result induction Even though results suggested ALVAC does elicit Ag-specific CTLs, immunization other Ags efficiently Our data show capacity transgene-encoded critically depends on presence highly epitopes Ags. This consideration needs be taken into account respect design evaluation strategies vaccine.