Erythropoietin Promotes Glioblastoma via miR-451 Suppression.

摘要: Abstract Erythropoietin (EPO) is an erythropoiesis stimulating growth factor and hormone. EPO has been widely used in the treatment of chronic renal failure, cancer, chemotherapy-related anemia for three decades. However, many clinical trials showed that may be associated with tumorigenesis cancer progression. able to cross blood–brain barriers, this lead increased possibility central nervous system tumors such as glioblastoma. Indeed, promotes glioblastoma invasion animal studies. Additionally, increases cell survival, proliferation, migration, invasion, chemoresistancy vitro. exact mechanisms progression induced by are not fully understood. Posttranscriptional gene regulation through microRNAs contribute EPO's cellular biological effects tumor Here, we aimed study whether suppressive microRNA, miR-451, counteracts positive on U87 human line. Migration were evaluated scratch assay transwell assay, respectively. We found decreased basal miR-451 expression cisplatin overexpression transfection its mimic significantly reversed these effects. Furthermore, ectopic inhibited own target genes, metalloproteinases-2 -9, which stimulated involved carcinogenesis processes, especially invasion. These findings suggest delivery useful adjuvant therapy addition chemotherapy should tested experimental models.