Foetal Rat Pancreas in Organ Culture: Effect of Corticosterone Concentrations on the Acinar and Islet Cell Components

摘要: Foetal rat pancreatic explants (20-day postcoitum) were grown in organ culture on medium enriched with serum and embryo extract containing various concentrations of corticosterone. Normal exocrine morphology was preserved. In addition, media amylase concentration remained high insulin suppressed. This is sharp contrast to incubated control without addition steroid which a rapid disappearance the acinar component selective proliferation islet cells noted. The magnitude these effects related steroid. Corticosterone effective preserving through 8–10 days vitro. Removal levels corticosterone from incubation after 4 resulted decrease fall explant cell mass. returned during following culture. Addition no increase statistically significant differences Media decreased additional incubation. However, corticosterone, even at 10.0 μg/ml, not depressing secretion as foetal adrenal co-culture. It proposed that corticosteroids are responsible for maintenance differentiated previously observed suggests may play an important role vivo morphogenesis cytodifferentiation. directly inhibit release beta