Molecular Identification of Tuber Species and Isolates by PCR-Based Techniques

摘要: Morphological features of fruit bodies and ectomycorrhizas allow the identification most truffle species. However, economically important truffles (e.g. Tuber aestivum / T. uncinatum; borchii/ magnatum ) are difficult to distinguish using these criteria. It is therefore required, when morphological traits unsufficient for discriminating, call additional markers isozymes DNA). DNA polymorphisms in genomes have been used generate molecular by PCR amplification specific regions random polymorphic (RAPD). Amplification internal transcribed spacer (ITS) intergenic (IGS) ribosomal combined with endonuclease digestion yielded unique diagnostic patterns European rDNA polymorphism was not high enough uncinatum. This approach revealed a low intraspecific variation within melanosporum. The spacers can be RFLP, allowing species typed less than day immediately applicable epidemiology. has further assessed RAPD technique. interspecific genomic variability already observed PCR-RFLP confirmed. In addition, large variations found all species, except melanosporum brumale. Molecular techniques provide an efficient tool, on one hand typing fruiting food industry, mycelium patenting purposes as well mycorrhiza checking inoculated seedlings other tracking fate mycorrhizal truffieres forest ecosystems.