Grouping and identification of Tuber species using RAPD markers
作者： Delphine Gandeboeuf , C. Dupré , G. Chevalier , P. Roeckel-Drevet , P. Nicolas
DOI: 10.1139/B97-005
关键词: UPGMA 、 Biology 、 RAPD 、 Tuber brumale 、 Taxon 、 Botany 、 Genetic variation 、 Interspecific competition 、 Polymerase chain reaction 、 Tuber melanosporum
摘要: Mycorrhizal fungi of the genus Tuber are classified by morphological characters that allow differentiation most species. However, some economically important species difficult to differentiate on grounds. When traits not sufficient discriminate between taxa, other markers needed. Genetic variation fruit bodies 12 taxa was studied random amplified polymorphic DNA (RAPD) technique. High interspecific variability observed Moreover, infraspecific occurred in all species, except brumale s.L, melanosporum, and magnatum. Southern hybridization patterns RAPD products various were used confirm data. Relationships among determined cluster analyses. UPGMA analyses revealed several main clusters a low genetic similarity taxa. These results indicate polymerase chain reaction useful for analysing wi...
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