Changes in the evolution of the antigenic profiles and morphology during coccoid conversion of Helicobacter pylori.

摘要: OBJECTIVES The significance of the coccoid forms H. pylori is still controversial and questions whether these are viable infective or degenerative open. We induced conversion from rod to studied morphological changes antigenic evolutions during this and, thereby, elucidated viability forms. METHODS strain (C001) used for Western blotting was isolated patient with gastric cancer. evolution by blotting, using different sera thirty patients known be culture positive. These were reveal total antigens cultured 2 days (100% rod) 15 (> 99% coccoid). After SDS-PAGE, 10% separating gel (rod coccoid), transblotting (Trans-Blot electrophoretic cell, Bio-Rad) taken onto a nitrocellulose membrane (Bio-Rad). Then, blots, human diluted at 1/100, developed color reaction goat serum anti-human IgG alkaline phosphatase BCIP. RESULTS profiles not changed in 46.7% (14/30 cases) 53.3% (16/30 conversion. Antigenic fractions protein band 120 kDa 35 kDa, detected coccus rest identical between which disappeared include CagA (120 kDa) porin, adhesin (35 kDa). U shaped day 7, doughnut 9 full 15. CONCLUSIONS results showed that retain cellular structures similar form, some (CagA porin) Therefore, form might represent one stages biological cycle.