Induction of microsomal aryl hydrocarbon (3,4-benzo(α)pyrene) hydroxylase and cytochrome P-450k in rat kidney cortex
作者： Robert Grundin , Sten Jakobsson , Dominick L. Cinti
DOI: 10.1016/0003-9861(73)90547-X
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摘要: Abstract Both the rat kidney cortex aryl hydrocarbon hydroxylase activity and cytochrome P-450 K are induced by benzo(α)pyrene treatment. Following a single injection of benzo(α)pyrene, maximal content occur at 24 hr, returning to control levels within 72–96 hr. Induction both enzyme hemoprotein is inhibited cycloheximide. The system localized in microsomal fraction, has an absolute requirement for NADPH molecular oxygen, pH optimum 7.4; linear with protein concentration up 0.8 mg time 20 min. follow same pattern inactivation increasing temperature. apparent m was 7.7 μ V increased fourfold above value. In presence laurate, substrate -dependent monooxygenase system, amount inhibition corresponded level present untreated microsomes. α-Naphthoflavone (10 −5 ), type I inducer (36) produced greater inhibitory effect on than (55% vs 20%). c or carbon monoxide markedly decreased activity. This evidence addition aforementioned characteristics suggests which differs from that rat.
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