Prognostic significance of reverse transcriptase-polymerase chain reaction measurement of carcinoembryonic antigen mRNA levels in tumor drainage blood and peripheral blood of patients with colorectal carcinoma.

摘要: BACKGROUND The objective of this study was to assess the prognostic value detecting circulating tumor cells in drainage blood and peripheral patients with colorectal carcinoma. METHODS A total 53 advanced carcinoma were studied. Tumor samples that obtained during surgery examined for carcinoembryonic antigen (CEA) mRNA using reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS In spiking experiments, assay indicated a sensitivity limit 1 cell per 106 cells. CEA detected 36 (68%) 18 (34%). The rate detection both significantly higher lymph node metastasis, lymphatic invasion, or vascular invasion (P < 0.05). Among those who underwent potentially curative resection, 2-year disease free survival whose negative better than showed positivity either sample (tumor blood: 100% vs. 57%; P 0.05; 74% 50%; 0.01), although follow-up thus far has been only 9–30 months. CONCLUSIONS The presence is useful indicator cells, it one determinant factors carcinoma. Moreover, absence found be superior marker compared blood. Cancer 2000;89:970–6. © 2000 American Society.