Usefulness of p16ink4a, ProEX C, and Ki-67 for the Diagnosis of Glandular Dysplasia and Adenocarcinoma of the Cervix Uteri

摘要: Although the diagnostic criteria of in-situ and invasive adenocarcinomas cervix uteri are well established, differentiation from benign mimics may be difficult morphologic features precursors endocervical adenocarcinoma still debated. In this study, we evaluated usefulness p16ink4a (p16), ProEX C, Ki-67 for diagnosis its precursors. Immunohistochemistry with p16, was performed in 82 glandular lesions including 15 adenocarcinomas, 29 situ (AIS), 22 non-neoplastic samples, 16 cases dysplasia (GD), which showed significant nuclear abnormalities but did not meet AIS. The immunohistochemical expression pattern scored according to percentage stained cells (0, 1+, 2+, 3+ when 0% 5%, 6% 25%, 26% 50%, more than 50% were stained, respectively) each antibody. p16 at least focally expressed (1+ or more) 14 all AIS 7 negative samples. C both 1+ 8 6 respectively. Of GD 15, 14, Ki-67, score differences between neoplastic samples highly marker (P<0.001); however, distribution by differed significantly only (P=0.006) which, compared other markers, often a pattern. Our study shows that helpful also improve accuracy GD. particularly problematic cases, combination proliferation can provide additional help interpretation these lesions.