Gene expression differences between the microsatellite instability (MIN) and chromosomal instability (CIN) phenotypes in colorectal cancer revealed by high-density cDNA array hybridization.

摘要: Two distinct pathways of tumorigenesis exist in sporadic colorectal cancer. The microsatellite instability pathway (MIN), which is characterized by widespread due to aberrant mismatch repair machinery, accounts for 15% all cancers. chromosomal (CIN) phenotype, 85% cancers, gross lesions but the underlying mechanism remains unclear. We have addressed differences gene expression between MIN and CIN cancer phenotypes vitro use high density cDNA filters compare patterns cell-lines yielding a panel 73 consistently differentially expressed genes. Nine these genes were subjected confirmatory analysis independent methods, six confirmed as being expressed; PLK, RanBP2 CCNA2 overexpressed lines while BTF3, H2AZ PTPD1 lines. These are involved diverse processes, such maintenance chromatin architecture, DNA-damage checkpoint cell cycle regulation, may contribute phenotypes.