Different angiogenic phenotypes in primary and secondary glioblastomas.

摘要: Primary and secondary glioblastomas (pGBM, sGBM) are supposed to evolve through different genetic pathways, including EGF receptor PDGF its thus genes that involved in tumor-induced angiogenesis. However, whether other angiogenic cytokines also differentially expressed these glioblastoma subtypes is not known so far, but this knowledge might be important optimize an antiangiogenic therapy. Therefore, we studied the expression of several cytokines, VEGF-A, HGF, bFGF, PDGF-AB, PDGF-BB, G-CSF GM-CSF pGBMs sGBMs as well gliomas WHO III, precursor lesions sGBMs. In tumor tissues, all was observed albeit with marked differences concerning intensity distribution pattern. Quantification supernatant 30 tissue-corresponding glioma cultures revealed a predominant VEGF-A significantly higher levels PDGF-AB HGF bFGF were determined nearly no GBM subtype or malignancy-related differences. Interestingly, especially produced less frequently by cells. secretion occurred together increased number simultaneously secreted correlated worse patient prognosis may represent more aggressive phenotype. Finally, confirmed independent contribution each tumor-derived cytokine analyzed vascularization. Our data indicate optimal therapy require targeting multiple pathways seem differ markedly