In vitro effect of homocysteine on some parameters of oxidative stress in rat hippocampus.

摘要: Homocystinuria is an inherited metabolic disease characterized biochemically by increased blood and brain levels of homocysteine caused severe deficiency cystathionine beta-synthase activity. Affected patients present mental retardation, seizures, atherosclerosis. Oxidative stress plays important role in the pathogenesis many neurodegenerative vascular diseases, such Alzheimer's disease, stroke, However, mechanisms underlying neurological damage characteristic homocystinuria are still poorly understood. To evaluate involvement oxidative on dysfunction homocystinuria, we measured thiobarbituric acid reactive substances (TBARS), index lipid peroxidation, total radical-trapping antioxidant potential (TRAP) enzyme activities (superoxide dismutase, catalase, glutathione peroxidase) rat hippocampus absence (controls) or presence (10-500 microM) vitro. We demonstrated that significantly increases TBARS decreases TRAP, both a dose-dependent manner, but did not change enzymes. Our results suggest involved homocystinuria. further studies necessary to confirm extend our findings human condition also determine whether therapy may be benefit these patients.