Effects of platelet-derived growth factor and transforming growth factor-beta 1 on the synthesis of a large versican-like chondroitin sulfate proteoglycan by arterial smooth muscle cells.

摘要: Platelet-derived growth factor (PDGF) and transforming factor-beta 1 (TGF-beta 1) increase [35S]sulfate incorporation into proteoglycan (PG) by monkey arterial smooth muscle cells but have opposite effects on cell proliferation. The combination of these two regulatory peptides has an additive effect PG synthesis no time course sulfate after stimulation indicates that both factors cause maximal glycosaminoglycan chains 12-18 h. is most affected a large CSPG (Mr approximately 1.2 x 10(6)) which can be immunoprecipitated antibody against versican, synthesized human skin fibroblasts. hydrodynamic size this molecule increases PDGF TGF-beta stimulation, the core glycoprotein 450,000) remains same. Treatment with either leads to in amount for PG. This correlates steady state level mRNA identified hybridization cDNA encoding versican. also chain length accounting greater stimulation. In contrast, not changes composition attached doubling ratio chondroitin 6-sulfate 4-sulfate. These results indicate although net versican like CSPG, they differ their structure chains. post-translational modifications may relate cells.