Simultaneous determination of synthetic dyes in foodstuffs and beverages by high-performance liquid chromatography coupled with diode-array detector

摘要: Abstract Synthetic dyes are added to food and drinks restore their original appearance when color is affected by processing, storage, packaging distribution. Furthermore colors used make more visually attractive consumers. The EU Directive 1994/36/CE lists the permitted substances that can be in foodstuffs. In order investigate content of some non-permitted drinks, a sensitive helpful method has been developed determine simultaneously seventeen synthetic colorants high-performance liquid chromatography coupled with diode-array detector solid matrices beverages. Substances involved were azorubine (E122), amaranth (E123), cochineal red A (E124), 2G (E128), allura (E129), azocarmine B (AZO B), G G), ponceau 2R (P2R), 6R (P6R), tartrazine (E102), sunset yellow (E110), quinoline (E104), orange II (OR II), metanil (MY), patent blue V (E131), indigo carmine (E132) brilliant FCF (E133). Solid extracted water–alcohol mixture, cleaned up on polyamide SPE cartridge eluted basic methanol solution. Otherwise simple dilution filtration samples for drinks. validated according Regulation (2004/882/CE) could applied concentration range between 5 300 mg kg−1 (5–100 mg l−1 drinks) depending dye. accuracy (precision trueness) specificity assessed. During years 2009–2011 many detection analyzed. Most them fresh fishery products which use colorant banned.