Truncation of the human EGF receptor leads to differential transforming potentials in primary avian fibroblasts and erythroblasts.

摘要: Abstract The transforming capacity of the normal and mutant human EGF receptor (EGFR) was investigated in primary chicken cells. In fibroblasts, both N- C-terminal truncations resulted a weak, additive oncogenic activity. However, not even double caused v-erbB-like phenotype. Upon EGF-binding, on other hand, C-terminally truncated EGFRs resembled v-erbB their fibroblast potential. erythroblasts, N-terminal truncation sufficient to induce constitutive self-renewal, which enhanced by deletion 32 amino acids but abolished larger 202 acids. contrast EGFR, lacking conferring erythropoietin independence for spontaneous differentiation transformed erythroblasts. Our results indicate that domain EGFR is non-essential transformation, seems be crucial self renewal induction specificity function