Screening for hepatitis B virus in healthy blood donors by molecular DNA hybridization analysis.
作者： C F Sun , C C Pao , S Y Wu , Y F Liaw
DOI: 10.1128/JCM.26.9.1848-1852.1988
关键词: Hepatitis B virus DNA polymerase 、 Hepatitis B 、 Hepatitis B virus 、 Antibody 、 HBsAg 、 Southern blot 、 Virology 、 Antigen 、 Biology 、 Hepadnaviridae 、 Molecular biology
摘要: A DNA molecular hybridization technique employing a purified adw subtype hepatitis B virus (HBV) cloned of 3.2 kilobase pairs as probe was used to screen for the presence HBV in blood samples collected from 486 apparently healthy donors. Eighteen 104 (17.3%) surface antigen (HBsAg) carriers and 7 382 (1.8%) HBsAg-negative individuals had circulating their sera. Among seven who were positive but negative HBsAg, three antibodies against both HBsAg (anti-HBsAg) core antigen, one only anti-HBsAg, anti-hepatitis e two all above markers. The results suggest that absence otherwise may not be enough ensure lack HBV.
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