Pituitary Adenylate Cyclase-Activating Polypeptide is Protective Against Oxidative Stress in Human Retinal Pigment Epithelial Cells
作者： Laszlo Mester , Krisztina Kovacs , Boglarka Racz , Izabella Solti , Tamas Atlasz
DOI: 10.1007/S12031-010-9427-9
关键词: Oxidative stress 、 Neuroprotection 、 Apoptosis 、 Cell biology 、 Excitotoxicity 、 Cell culture 、 Adenylate kinase 、 Endocrinology 、 Biology 、 Retinal 、 In vitro 、 Internal medicine
摘要: Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with potent neurotrophic and neuroprotective effects. We have previously shown that PACAP protects against several types of retinal injuries in vivo, including ischemia, glutamate-induced excitotoxicity, UV A-induced lesion, diabetic retinopathy. also activates antiapoptotic pathways inhibits proapoptotic signaling lesions vivo. receptors been identified on the pigment epithelial cells has to inhibit interleukin secretion from cells. It not known, however, whether protective these Human (ARPE-19 cell line) were exposed vitro oxidative stress by hydrogen peroxide. Cell survival was decreased stress, which could be significantly dose-dependently attenuated 10 pM–1 μM treatment, as MTT viability test. The effect blocked receptor antagonist PACAP6-38. In addition, flow cytometry JC-1 assay revealed stress-induced apoptosis treatment. summary, results show, for first time,
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