Proliferation and osteogenic differentiation of amniotic fluid-derived stem cells.

摘要: Human amniotic fluid-derived stem cells (hAFCs) are pluripotent fetal capable of differentiating into multiple lineages, including cell types each the three embryonic germ layers. Proper differentiation and maintenance pluripotency, defining characteristics cells, regulated not only by themselves but also their microenvironment. Furthermore, physical culture materials, such as material elasticity, influence results differentiation. We investigated osteogenic efficiency hAFCs cultured on materials with different elasticities that were grafted extracellular matrix-derived oligopeptides. Polyvinyl alcohol-co-itaconic acid (PV) hydrogels prepared controlling crosslinking intensity, resulting PV without matrix (ECM)-derived Specific ECM-derived oligopeptides used to maintain pluripotency AFCs determined evaluation gene expression (Sox2 Oct4). The hAFCs, oligopeptides, was analyzed alkaline phosphatase activity, Alizarin Red S staining, von Kossa staining. Unmodified induced osteoblast high efficiency. conclude interacting tended an undifferentiated state.