Evaluation of an ELISA for Detection of Ovine Progressive Pneumonia Antibodies using a Recombinant Transmembrane Envelope Protein
作者： Jimmy Kwang , Jim Keen , Randall C. Cutlip , E. Travis Littledike
DOI: 10.1177/104063879300500208
关键词: Transmembrane protein 、 Ovine lentivirus 、 Coating antigen 、 Biology 、 Virology 、 Ouchterlony double immunodiffusion 、 Antigen 、 Molecular biology 、 Antibody 、 Recombinant DNA 、 Ovine progressive pneumonia
摘要: An enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant protein corresponding to the N'-terminal hydrophilic region of transmembrane glycoprotein (TM) ovine lentivirus. This reproducibly detected antibodies in sera from 207 212 progressive pneumonia (OPP) virus-infected sheep, and TM ELISA accurately identified 26% (35 vs. 9) more seropositive samples than did agar gel immunodiffusion test when applied 100 an infected flock. also yielded no false-positive results 14 true negative sera. Results these experiments were further confirmed by p25 Western blot assay. A single antigen, as coating antigen ELISA, can be used successfully for detection OPP animals improve sensitivity specificity diagnosis.
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