Cloning and characterization of HIV-1-inducible astrocyte elevated gene-1, AEG-1

摘要: We presently describe the full-length cloning and functional characterization of an HIV-1-inducible gene, astrocyte elevated gene (AEG)-1. Additionally, a novel method is outlined for producing tag-free recombinant protein in baculovirus system its use AEG-1 protein. mRNA expressed ubiquitously with higher expression tissues containing muscular actin increased astrocytes infected HIV-1 or treated gp120 tumor necrosis factor (TNF)-alpha. The encodes single pass transmembrane predicted molecular mass 64-kDa pI 9.3 that predominantly localizes endoplasmic reticulum perinuclear region. Ectopic inhibits excitatory amino acid transporter 2 (EAAT2) promoter activity potential to promote glutamate excitotoxicity consequently HIV-1-associated dementia (HAD). subsets breast carcinomas, malignant gliomas melanomas it synergizes oncogenic Ha-ras enhance soft agar colony forming ability non-tumorigenic immortalized melanocytes, documenting promoting activity. may affect progression multiple cell lineages by augmenting transformed phenotype and/or inducing glioma. In these contexts, AEG-1, contribute brain abnormalities, including HAD formation, both common distinct mechanisms.