Expression of P-Selectin at Low Site Density Promotes Selective Attachment of Eosinophils Over Neutrophils
作者： Bruce S. Edwards , Mark S. Curry , Hisashi Tsuji , David Brown , Richard S. Larson
DOI: 10.4049/JIMMUNOL.165.1.404
关键词: Eosinophil 、 Cell biology 、 Biology 、 Chinese hamster ovary cell 、 Immunology 、 Glycoprotein 、 P-selectin 、 Endothelial stem cell 、 Selectin 、 Adhesion 、 Eosinophilia
摘要: The selective interaction of neutrophils with E-selectin and eosinophils P-selectin has been previously reported, but the relevance selectin site density fluid shear not studied in detail. We have developed a new approach to examine these interactions cell suspensions that integrates an on-line cone-plate viscometer flow cytometer. find both use glycoprotein ligand-1 form stable conjugates Chinese hamster ovary transfectants, preferential adhesion eosinophils. Further, difference between is magnified at expression levels below ∼20 sites/μm 2 , range likely be relevant endothelial conditions associated eosinophilia. unique behavior retained over rates ranging from 100 1500/s low shear. Results parallel-plate chamber assays suggest eosinophil reflects enhanced efficiency initial PSGL-1 bond formation rather than ability mediate rolling longer duration on low-density substrates. These differences may account part for increase accumulation allergic diseases.
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