MiR-21 mediates the radiation resistance of glioblastoma cells by regulating PDCD4 and hMSH2

摘要: The purpose of this study was to investigate the molecular mechanism by which miR-21 and its target genes mediate radiation resistance glioblastoma cells. Real-time PCR employed detect expression in normal brain tissues, tissues cell lines (A172, T98G U87MG). cells were transfected with anti-miR-21 oligonucleotides, or plasmids containing PDCD4 hMSH2 (PDCD4-pcDNA3 hMSH2-pcDNA3). survival curve obtained sensitivity radiation. Cell apoptosis measured using Caspase-3/7 kit cycle flow cytometry. Western blotting performed miR-21-inhibiting results showed that conversely associated sensitivity. Over-expression resulted resistance, while knockdown led higher glioblastma After knockdown, significantly increased G(2) phase arrest more significant. In addition, endogenous hMSH2, contributed findings suggested may against via hMSH2. MiR-21 be used as potential targets for clinical radiotherapy sensitization future.