Modulation of the invasive phenotype of human colon carcinoma cells by organ specific fibroblasts of nude mice.

摘要: We examined whether fibroblasts from subcutaneous, colon or lung tissues of nude mice influence the invasive potential highly metastatic human carcinoma KM12SM cells. Primary cultures mouse skin, and were established. Invasive cells cultured alone with fibroblasts. Growth properties evaluated as well their production gelatinase activity. able to grow on monolayers all three fibroblast but did not invade through skin The conditioned media cocultured for presence type IV collagenase (gelatinase). growing plastic produced significant levels latent active forms 64 kDa collagenase, whereas cocultivated not. In contrast, squamous cell A431 when fibroblasts, a tissue they invaded completely penetrated. Incubation in serum-free medium containing recombinant interferon-beta (fibroblast interferon) was associated reduction Since by cancer is specifically inhibited data suggest that organ-specific can