Prevalence of Exon 15 BRAF Mutations in Primary Melanoma of the Superficial Spreading, Nodular, Acral, and Lentigo Maligna Subtypes
作者： Julie Lang , Rona M. MacKie
DOI: 10.1111/J.0022-202X.2005.23833.X
关键词: Pigmentation disorder 、 Cancer research 、 Melanocyte 、 Melanoma 、 Malignant transformation 、 Mucosal melanoma 、 Lentigo maligna 、 Biology 、 Pathology 、 Exon 、 Mutation
摘要: DNA was extracted from 52 thick primary melanomas and mutations sought in exon 15 of the BRAF (v-raf murine sarcoma viral oncogene homolog B1) gene using denaturing high performance liquid chromatograph (dHPLC) fragment analysis, sequencing, allele-specific PCR. Exon were found 13 (25%) melanomas. These comprised five 17 (29%) superficial spreading melanomas, three 11 (27%) nodular two (15%) acral lentiginous one (100%) mucosal melanoma 10 (20%) lentigo maligna In common with other groups, our findings show a relative concentration mutation but add further evidence that this not necessary for malignant transformation melanocyte.
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