Potential usefulness of detecting cyclooxygenase 2 messenger RNA in feces for colorectal cancer screening

摘要: Abstract Background & Aims: Cyclooxygenase 2 (COX-2) is overexpressed frequently in aerodigestive tumors, especially colorectal tumors. Therefore, it may be a suitable biomarker for cancer (CRC) screening. We performed pilot study of whether detecting COX-2 expression fecal RNA enables us to discriminate between patients with and without CRC. Methods: The cohort included 29 CRC, 22 control neoplastic disease the colon or rectum. was isolated from routinely collected stool samples using modified method. levels carcinoembryonic antigen (CEA) were determined by nested reverse-transcription polymerase chain reaction (RT-PCR). Results: sensitivity specificity assay CRC 90% (95% confidence interval [CI], 73%–98%) 100% CI, 85%–100%), respectively, whereas those CEA 88%–100%) 5% 2%–23%), respectively. messenger (mRNA) detected 3 4 Dukes' stage A, 13 14 B, 10 11 C D. mRNA 5 7 proximal 21 distal cancer. superior terms specificity, although both assays had high sensitivity. Conclusions: This These results suggest that would promising approach larger necessary assess specificity.