Optimization of the composition and pH of the mobile phase used for separation and determination of a series of quinolone antibacterials regulated by the European Union

摘要: To ensure the safety of human food European Union (EU) has set tolerance levels for quinolone compounds in animal products, so screening and confirmatory analytical methods are required monitoring these drugs. In this work, proportion organic modifier pH acetonitrile-water mixtures used as mobile phases were optimized separation a group quinolones. Linear solvation energy relationship (LSER) formalism based on single solvent polarity parameterE T N was to predict chromatographic behaviour function amount acetonitrile phase. Correlation between retention aqueous-organic also been optimize mobile-phase pH. The phase linear gradient starting from 18∶82 (v/v) acetonitrileacetate + formate buffer, 2.5. Quality data determined satisfactory. method detection limit approximately 10 ng mL−1 most quinolones studied. proposed is compatible with mass spectrometric substances.