Dissociation of differentiation and proliferation in the primary induction of cytolytic T lymphocytes by alloantigens.

摘要: The requirement for DNA synthesis in the induction of cytolytic T lymphocytes (CTL) by alloantigens has been investigated. C57BL/6 splenic cells purified passage on nylon wool columns were stimulated vitro mixed leukocyte culture (MLC) and assayed cytotoxicity against 51Cr-labeled target cells. With this system, CTL activity was detectable after 24 hr MLC reached high levels 48 hr. Addition cytosine arabinoside (ARA-C) or hydroxyurea to such cultures at concentrations that sufficient inhibit greater than 98% did not reduce measured hr; however, increase occurred between control strongly reduced (or abolished) these drugs. Velocity sedimentation analysis activated presence ARA-C further revealed precursor had enlarged into medium- large-sized under conditions. These studies provide direct evidence primary can be dissociated a differentiation step, which occurs within absence is accompanied blast transformation, subsequent proliferation.