Differential targets of CpG island hypermethylation in primary and metastatic head and neck squamous cell carcinoma (HNSCC).

摘要: Head and neck squamous cell carcinomas (HNSCC) often metastasise to the cervical lymph nodes. It is known for HNSCC as well other cancers that progression from normal tissue primary tumour finally metastatic characterised by an accumulation of genetic mutations. DNA methylation, epigenetic modification, can result in loss gene function cancer, similar mutations such deletions point We have investigated methylation phenotypes both tumours 13 patients using restriction landmark genomic scanning (RLGS). With this technique, we were able assess status average nearly 1300 CpG islands each tumour. observed number hypermethylated significantly greater than what found overall, but not every patient. Interestingly, data also clearly show many loci methylated a patient's are no longer same Thus, even though methylate proportion do tumours, they so at different subsets loci. These unanticipated variability state HNSCCs within discuss two possible explanations how events might arise between person.