CpG island hypermethylation profiling of lung cancer using restriction landmark genomic scanning (RLGS) analysis.
作者： Jong Park , Romulo Martin Brena , Mike Gruidl , Jun Zhou , Tim Huang
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摘要: Lung cancer remains the leading cause of related mortality, accounting for almost one-third deaths in men and one-fourth women; 160,440 lung are expected 2004. Survival from depends mainly upon stage at presentation. As localized tumors generally do not symptoms, disease is usually diagnosed symptomatic patients advanced stages when prognosis poor. a result, overall 5-year survival rate only 15%. It well known that epigenetic alterations such as DNA methylation CpG dinucleotides located islands within regulatory (promoter) regions genes associated with transcriptional silencing cancer. Promoter hypermethylation critical pathway could identify potential biomarkers risk. Our goal this study to novel hypermethylated We have investigated profiles samples 14 paired tumor adjacent normal tissues resected same individuals using restriction landmark genomic scanning (RLGS). assess status an average 2,012 each tumor. identified 162 differentially methylated loci where were but non-cancer tissues. Among sites differential methylation, detected least one tumor/normal pair, 21 reported previously tissue.
utsystem.edu参考文章(42)
Ada Chen, Hiun Suk Chae, Guoren Deng, Joe Hong, Young S. Kim, Methylation of CpG in a Small Region of the hMLH1 Promoter Invariably Correlates with the Absence of Gene Expression Cancer Research. ,vol. 59, pp. 2029- 2033 ,(1999)
Stephen B. Baylln, James G. Herman, Jeremy R. Graff, Paula M. Vertino, Jean-Pierre Issa, ALTERATIONS IN DNA METHYLATION : A FUNDAMENTAL ASPECT OF NEOPLASIA Advances in Cancer Research. ,vol. 72, pp. 141- 196 ,(1998) , 10.1016/S0065-230X(08)60702-2
J. Michael Glendening, Christina M. Bender, Jane W. Fountain, Peter A. Jones, José F. Flores, Nicholas K. Hayward, Mark L. Gonzalgo, Graeme J. Walker, Graeme J. Walker, Edward H. You, Low frequency of p16/CDKN2A methylation in sporadic melanoma: comparative approaches for methylation analysis of primary tumors. Cancer Research. ,vol. 57, pp. 5336- 5347 ,(1997)
Etzioni Ruth, Urban Nicole, Ramsey Scott, M Martin, Schwartz Stephen, Reid Brian, Radich Jerald, Anderson Garnet, Hartwell Leland, THE CASE FOR EARLY DETECTION Nature Reviews Cancer. ,vol. 3, pp. 243- 252 ,(2003) , 10.1038/NRC1041
Peter M. Warnecke, Paul C. Vincent, Marianne Frommer, Susan J. Clark, Clare Stirzaker, Douglas S. Millar, Cheryl L. Paul, Janet Harrison, Extensive DNA Methylation Spanning the Rb Promoter in Retinoblastoma Tumors Cancer Research. ,vol. 57, pp. 2229- 2237 ,(1997)
Timothy H.C. Hsiau, Yu Wei Leu, Farahnaz Rahmatpanah, Pearlly S. Yan, Andrew H.A. Hsiau, Joseph C. Liu, Huidong Shi, Tim Hui Ming Huang, Differential Distribution of DNA Methylation within the RASSF1A CpG Island in Breast Cancer Cancer Research. ,vol. 63, pp. 6178- 6186 ,(2003)
Benjamin F. Hankey, Lynn A. Ries, Brenda K. Edwards, The surveillance, epidemiology, and end results program: a national resource. Cancer Epidemiology, Biomarkers & Prevention. ,vol. 8, pp. 1117- 1121 ,(1999)
DJ Smiraglia, LT Smith, JC Lang, LJ Rush, Z Dai, DE Schuller, C Plass, Differential targets of CpG island hypermethylation in primary and metastatic head and neck squamous cell carcinoma (HNSCC). Journal of Medical Genetics. ,vol. 40, pp. 25- 33 ,(2003) , 10.1136/JMG.40.1.25
Jeffrey A Tsou, Jeffrey A Hagen, Catherine L Carpenter, Ite A Laird-Offringa, DNA methylation analysis: a powerful new tool for lung cancer diagnosis Oncogene. ,vol. 21, pp. 5450- 5461 ,(2002) , 10.1038/SJ.ONC.1205605
Frank Larsen, Glenn Gundersen, Rodrigo Lopez, Hans Prydz, CpG islands as gene markers in the human genome. Genomics. ,vol. 13, pp. 1095- 1107 ,(1992) , 10.1016/0888-7543(92)90024-M