Cytoprotective role of taurine in a renal epithelial cell culture model

摘要: Taurine (TAU) is a sulfur-containing amino acid that has been shown to decrease during aging and believed be important for cytoprotection. A in TAU could exacerbate the accumulation of free radical-induced damage may lead cell death process. We have previously directly inhibits dopamine (DA) (-)-3-(3,4-dihydroxyphenyl)-L-alanine (L-dopa) oxidation. Experiments were conducted establish cytoprotective role TAU. Porcine renal epithelial cells treated 1 hr with iron catecholamines (L-dopa DA) produce cytotoxicity by radical quinone mechanism absence presence 10 or 20mM Viability assays, protein, DNA measurements performed after 24hr recovery period. In some experiments, extracted immediately insult DA content using high performance liquid chromatography electrochemical detection. Catecholamine-induced caused 50% loss viability, provided significant protection from maintained functional integrity cells. Photomicrographs showed attenuation swelling Pretreatment 1mM followed exposure L-dopa moderate but non-significant increase survival. These data conclusively show can play LLC-PK(1) culture model.