Balance between activation and inhibition of matrix metalloproteinase-2 (MMP-2) is altered in colorectal tumors compared to normal colonic epithelium.
作者： Deborah L. Ornstein , Kenneth H. Cohn
关键词: Epithelium 、 Cancer research 、 Metastasis 、 Matrix metalloproteinase 、 Malignant transformation 、 Intestinal mucosa 、 Matrix metalloproteinase inhibitor 、 Colorectal cancer 、 Biology 、 Pathology 、 Carcinoma
摘要: Matrix metalloproteinase-2 (MMP-2) is overexpressed in human cancers and facilitates tumor growth metastasis. It synthesized as an inactive proenzyme that activated by membrane-type matrix metalloproteinase-1 (MT1-MMP) inhibited tissue inhibitor of (TIMP-2). We hypothesized there imbalance between the expression TIMP-2 MMP-2 MT1-MMP favors activation malignant colon tumors compared to normal colonic tissue. Specimens adjacent mucosa were obtained from 22 patients at time surgical resection. MMP-2, MT1-MMP, RNA transcripts measured each sample using a quantitative reverse transcriptase polymerase chain reaction assay. observed levels significantly elevated (P = 0.039). In addition, TIMP-2:MMP-2 ratio was twofold lower 0.001) TIMP-2:MT1-MMP 1.5-fold 0.003) mucosa. These results suggest balance genes activate inhibit shifted toward tumors. The abnormal gene products regulate activity may be important early step transformation cancer provide useful target for new chemoprevention adjuvant treatment strategies.
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