Quantification of Ultrasonic Scattering Properties of In Vivo Tumor Cell Death in Mouse Models of Breast Cancer.

摘要: INTRODUCTION: Quantitative ultrasound parameters based on form factor models were investigated as potential biomarkers of cell death in breast tumor (MDA-231) xenografts treated with chemotherapy. METHODS: Ultrasound backscatter radiofrequency data acquired from MDA-231 cancer tumor–bearing mice (n = 20) before and after the administration chemotherapy drugs at two frequencies: 7 MHz 20 MHz. Radiofrequency spectral analysis involved estimating coefficient regions interest center tumor, to which fitted, resulting estimates average scatterer diameter acoustic concentration (AAC). RESULTS: The ∆AAC parameter extracted spherical Gaussian model was found be most effective biomarker (at lower frequency range, r2 0.40). At both frequencies, AAC tumors increased significantly (P .026 .035 low high respectively) 24 hours treatment compared control tumors. Furthermore, stepwise multiple linear regression low-frequency revealed that a multiparameter quantitative strongly correlated determined histologically posttreatment (r2 0.74). CONCLUSION: model–based scattering can potentially used track extent clinically relevant frequencies (7 MHz). 20-MHz results agreed previous findings related intensity (i.e., AAC) death. suggested that, addition ∆AAC, biological features including heterogeneity initial volume important factors prediction response.