Assessment of tumor response to radiation and vascular targeting therapy in mice using quantitative ultrasound spectroscopy

摘要: Purpose: It is now recognized that the tumor vasculature in part responsible for regulating responses to radiation therapy. However, extent which radiation-based vascular damage contributes cell death remains unknown. In this work, quantitative ultrasound spectroscopy (QUS) methods were used investigate acute of tumors treatments. Methods: Tumor xenografts (MDA-MB-231) treated with single doses 2 or 8 Gy alone, combination pharmacological agents modulate radiosensitivity. The midband fit, slope, and 0-MHz intercept QUS parameters obtained from a linear-regression fit averaged power spectrum frequency-dependent backscatter quantify following treatment administration. Power spectrums extracted raw volumetric radio-frequency data before 24 h These have previously been correlated death. Staining using situ end labeling, carbonic anhydrase 9 cluster differentiation 31 sections assess death, oxygenation, distributions, respectively. Results: Results indicate significant parameter increases 3.2 ± 0.3 dBr 5.4 0.5 combined antiangiogenic agent Sunitinib, respectively. contrast, alone demonstrated increase 4.4 at only. Preadministration basic fibroblast growth factor, an endothelial radioprotector, acted minimize response large radiation. Immunohistochemical analysis was general agreement findings; R2 0.9 observed when quantified changes fit. Conclusions: Results presented study confirm linked effect high Overall, previous reports suggesting regulated by vascular-driven response. Data also suggest Sunitinib may enhance radiosensitivity through remodeling process, be sensitive tissue properties associated remodeling. Finally, work demonstrates ability monitor strategies.