Activation of Wnt/β-catenin/GSK3β signaling during the development of diabetic cardiomyopathy.

摘要: Abstract Background As Wnt/β-catenin/glycogen synthase kinase 3β (GSK3β) signaling has been implicated in myocardial injury and diabetic cardiomyopathy (DCM) is a major part of cardiovascular complications, we therefore investigated the alterations Wnt/β-catenin/GSK3β during development DCM. Methods The rat model diabetes mellitus (DM) was established using single intraperitoneal injection streptozotocin (STZ, 60 mg/kg). were determined 4, 8, 12 weeks following DM Western blotting, immunohistochemistry, quantitative real-time reverse transcriptase polymerase chain reaction. Cardiac pathology changes evaluated hematoxylin eosin, Masson trichromatic, terminal dUTP nick-end labeling staining. Results Histological analyses revealed that induced significant progressive cardiomyocyte apoptosis. protein mRNA levels Wnt2, β-catenin, c-Myc progressively increased DM. expression T-cell factor 4 phosphorylated GSK3β on Ser9 increased. However, endogenous Wnt inhibitor Dickkopf-1 after STZ then decreased as DCM developed. Conclusion pathway activated Further investigation into role will functionally find novel therapeutic target for