Wnt2 is a direct downstream target of GATA6 during early cardiogenesis.
作者： Alexander Alexandrovich , Matthew Arno , Roger K. Patient , Ajay M. Shah , John A. Pizzey
DOI: 10.1016/J.MOD.2006.02.002
关键词:
摘要: The GATA4, 5 and 6 subfamily of transcription factors are potent transactivators expressed within the precardiac mesoderm. However, little is known immediate downstream targets GATA-factor regulation during earliest stages cardiogenesis. Using P19-CL6 embryonal carcinoma (EC) cell line as an in vitro model cardiogenesis, we show that GATA6 most abundantly GATA presumptive cardiac cells. Consequently, performed a microarray screen comparing mRNA from control EC cells, early differentiation pathway, with those which had been overexpressed. These studies identified 103 genes whose expression changed significantly this was verified representative array these by real-time RT-PCR. We one genes, Wnt2, mirrors vivo. In addition, its upregulation differentiating cells mediated direct binding GATA-factor(s) to cognate Wnt2 promoter, suggesting target
elsevier.com
sci-hub.se 参考文章(63)
C. Macneill, J. B. E. Burch, R. E. Poelmann, C. Mueller, A. C. Laverriere, T. Evans, GATA-4/5/6, a subfamily of three transcription factors transcribed in developing heart and gut. Journal of Biological Chemistry. ,vol. 269, pp. 23177- 23184 ,(1994) , 10.1016/S0021-9258(17)31636-8
Frédéric Charron, Pierre Paradis, Odile Bronchain, Georges Nemer, Mona Nemer, COOPERATIVE INTERACTION BETWEEN GATA-4 AND GATA-6 REGULATES MYOCARDIAL GENE EXPRESSION Molecular and Cellular Biology. ,vol. 19, pp. 4355- 4365 ,(1999) , 10.1128/MCB.19.6.4355
William D. Rees, Susan M. Hay, Nathalie C. Fontanier-Razzaq, Christos Antipatis, D. Nicholas Harries, Expression of the Growth Arrest Genes (GAS and GADD) Changes during Organogenesis in the Rat Fetus Journal of Nutrition. ,vol. 129, pp. 1532- 1536 ,(1999) , 10.1093/JN/129.8.1532
Hisayuki Yokoyama, Hideo Harigae, Shinichiro Takahashi, Satoru Takahashi, Kazumichi Furuyama, Mitsuo Kaku, Masayuki Yamamoto, Takeshi Sasaki, Regulation of YB-1 gene expression by GATA transcription factors Biochemical and Biophysical Research Communications. ,vol. 303, pp. 140- 145 ,(2003) , 10.1016/S0006-291X(03)00296-1
Hon S. Ip, Zhihua Tang, Min M. Lu, Michael S. Parmacek, Edward E. Morrisey, GATA-5: a transcriptional activator expressed in a novel temporally and spatially-restricted pattern during embryonic development. Developmental Biology. ,vol. 183, pp. 21- 36 ,(1997) , 10.1006/DBIO.1996.8485
Tessa Peterkin, Abigail Gibson, Matthew Loose, Roger Patient, The roles of GATA-4, -5 and -6 in vertebrate heart development. Seminars in Cell & Developmental Biology. ,vol. 16, pp. 83- 94 ,(2005) , 10.1016/J.SEMCDB.2004.10.003
Alison C. Brewer, Alexander Alexandrovich, Corey H. Mjaatvedt, Ajay M. Shah, Roger K. Patient, John A. Pizzey, GATA Factors Lie Upstream of Nkx 2.5 in the Transcriptional Regulatory Cascade That Effects Cardiogenesis Stem Cells and Development. ,vol. 14, pp. 425- 439 ,(2005) , 10.1089/SCD.2005.14.425
Jane Winer, Christopher Kwang S. Jung, Irene Shackel, P.Mickey Williams, Development and Validation of Real-Time Quantitative Reverse Transcriptase–Polymerase Chain Reaction for Monitoring Gene Expression in Cardiac Myocytesin Vitro Analytical Biochemistry. ,vol. 270, pp. 41- 49 ,(1999) , 10.1006/ABIO.1999.4085
Kevin McBride, Mona Nemer, Regulation of the ANF and BNP promoters by GATA factors: lessons learned for cardiac transcription. Canadian Journal of Physiology and Pharmacology. ,vol. 79, pp. 673- 681 ,(2001) , 10.1139/Y01-037
Amanda M. Masino, Teresa D. Gallardo, Celeste A. Wilcox, Eric N. Olson, R. Sanders Williams, Daniel J. Garry, Transcriptional Regulation of Cardiac Progenitor Cell Populations Circulation Research. ,vol. 95, pp. 389- 397 ,(2004) , 10.1161/01.RES.0000138302.02691.BE