Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli.
作者： Arkady Khodursky , Brian Peter , Patrick O. Brown , Justin Courcelle , Philip C. Hanawalt
DOI: 10.1093/GENETICS/158.1.41
关键词: Genetics 、 Biology 、 Repressor lexA 、 Repressor 、 Gene 、 SOS response 、 Gene expression profiling 、 Molecular biology 、 Escherichia coli 、 Mutant 、 SOS Response (Genetics)
摘要: The SOS response in UV-irradiated Escherichia coli includes the upregulation of several dozen genes that are negatively regulated by LexA repressor. Using DNA microarrays containing amplified fragments from 95.5% all open reading frames identified on E. chromosome, we have examined changes gene expression following UV exposure both wild-type cells and lexA1 mutants, which unable to induce under control. We report here time courses surrounding 26 documented lexA-regulated regions chromosome. observed 17 additional sites responded a lexA-dependent manner large number were upregulated lexA-independent although this was generally not more than twofold. In addition, transcripts either downregulated or degraded irradiation. These newly UV-responsive discussed with respect their possible roles cellular recovery
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