Expression and Processing of the [Pro2,Met13]Somatostatin-14 Precursor in the Intermediate Lobe of the Frog Pituitary
作者： Herve Tostivint , Didier Vieau , Nicolas Chartrel , Isabelle Boutelet , Ludovic Galas
关键词: Gene isoform 、 Pars intermedia 、 Northern blot 、 Pituitary gland 、 In situ hybridization 、 Anterior pituitary 、 Biology 、 Internal medicine 、 Neuropeptide 、 Somatostatin 、 Endocrinology
摘要: The biosynthesis of various hypothalamic neuropeptides has been previously reported in anterior pituitary cells but not intermediate lobe cells. We have recently demonstrated the occurrence two somatostatin isoforms frog brain, namely somatostatin-14 (SS1) and [Pro(2),Met(13)]somatostatin-14 (SS2). In present study, we demonstrate that gene encoding SS2 precursor (PSS2) is actively expressed pituitary. High concentrations PSS2 mRNA detected by Northern blot analysis situ hybridization pars intermedia distalis or nervosa. distribution PSS1- PSS2-derived peptides investigated immunohistochemistry using antisera directed against SS1 sequence 54-66 (PSS2(54-66)), respectively. antiserum stained only a network fibers neural few nerve processes lobe. contrast, PSS2(54-66) produced intense labeling melanotrope intermedia. Biochemical characterization immunoreactive materials extracts was performed combining high-performance liquid chromatography RIA detection. revealed existence major peaks exhibited same retention times as synthetic SS2. single peak likely corresponds to N-flanking peptide (PSS2(1-66)). study reveals selectively express fully process generate mature variant Taken together, these data provide first evidence hypophysiotropic neuropeptide intensely
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