First Report of Spring Viremia of Carp Virus (SVCV) in Wild Common Carp in North America

摘要: Abstract In spring 2002, an estimated 1,500 common carp Cyprinus carpio in Cedar Lake, northwestern Wisconsin, died over a 6-week period from late April through the first week June. Three moribund were necropsied and had signs consistent with viremia of (SVC) disease, including petechiae ecchymotic hemorrhages on skin, ascites, edematous kidney spleen. A virus was isolated fathead minnow cells shown to be rhabdovirus by electron microscopy. Immunoassay results indicated close serological relationship (SVCV). This confirmed reverse transcriptase–polymerase chain reaction assay subsequent analysis subsection glycoprotein gene. Immunocytochemistry serum neutralization tests that Lake isolate did not share complete antigenic identity European reference SVCV. Also, showed inhibition cytopathic effect after repeated subculture epithelioma papulosum cyprini ...