Quantification of conidial density of Aspergillus flavus and A. parasiticus in soil from almond orchards using real‐time PCR

摘要: Aims:  To design the Aspergillus flavus and parasiticus-specific primers a real-time PCR assay for quantification of conidial density in soil. Methods Results: Aspergillus A. DNA were designed based on internal transcribed spacer sequences to distinguish these two species from other fungal species. A method pathogen extraction directly soil samples was developed. Using primers, developed quantitatively determine each parasiticus soil, after generating corresponding standard curves. Known densities or significantly correlated with those tested PCR. Conclusions:  This study demonstrated applicability studies quantifying as inoculum sources. Significance Impact Study:  The flacus parasitic-specific can be widely used aflatoxin research. this provides potential approach quantify plant not only but also sources relation contamination environment, food feed commodities.