作者: Minoru Tada , Masao Omata , Shigenobu Kawai , Hiromitsu Saisho , Masao Ohto
DOI:
关键词: Cancer research 、 Pathology 、 CA19-9 、 Pancreatic disease 、 Pancreatic cancer 、 Pancreatitis 、 Gene mutation 、 Adenocarcinoma 、 Pancreas 、 Pancreatic juice 、 Biology
摘要: Pancreatic adenocarcinomas are known to have a high incidence of K- ras gene mutations. Differential diagnosis pancreatic cancer and chronic pancreatitis sometimes presents clinical dilemma. We recently developed highly sensitive specific polymerase chain reaction capable detecting 3–30 copies mutant genes harboring codon 12 single base changes in the presence 300,000 normal copies. Mutant were detected DNA purified from juice all 6 cases adenocarcinoma 1 case intraductal papillary neoplasms pancreas. In 2 other with adenocarcinoma, circulating metastatic cells peripheral blood. Activated not found three control (chronic choledocholithiasis) or blood two patients insulinomas. Notable conclusions this study that there can be significant levels shed tumor an even higher number juice. addition, different mutations some patients.