Detection of ras Gene Mutations in Pancreatic Juice and Peripheral Blood of Patients with Pancreatic Adenocarcinoma

作者: Minoru Tada , Masao Omata , Shigenobu Kawai , Hiromitsu Saisho , Masao Ohto

DOI:

关键词: Cancer researchPathologyCA19-9Pancreatic diseasePancreatic cancerPancreatitisGene mutationAdenocarcinomaPancreasPancreatic juiceBiology

摘要: Pancreatic adenocarcinomas are known to have a high incidence of K- ras gene mutations. Differential diagnosis pancreatic cancer and chronic pancreatitis sometimes presents clinical dilemma. We recently developed highly sensitive specific polymerase chain reaction capable detecting 3–30 copies mutant genes harboring codon 12 single base changes in the presence 300,000 normal copies. Mutant were detected DNA purified from juice all 6 cases adenocarcinoma 1 case intraductal papillary neoplasms pancreas. In 2 other with adenocarcinoma, circulating metastatic cells peripheral blood. Activated not found three control (chronic choledocholithiasis) or blood two patients insulinomas. Notable conclusions this study that there can be significant levels shed tumor an even higher number juice. addition, different mutations some patients.

参考文章(12)
Nicholas R. Lemoine, Sunjay Jain, Christine M. Hughes, Susan L. Staddon, Bernard Maillet, Peter A. Hall, Günter Klöppel, Ki-ras oncogene activation in preinvasive pancreatic cancer Gastroenterology. ,vol. 102, pp. 230- 236 ,(1992) , 10.1016/0016-5085(92)91805-E
Minoru Tada, Masao Omata, Masao Ohto, None, Clinical application of ras gene mutation for diagnosis of pancreatic adenocarcinoma Gastroenterology. ,vol. 100, pp. 233- 238 ,(1991) , 10.1016/0016-5085(91)90606-L
Quin Chou, Marion Russell, David E. Birch, Jonathan Raymond, Will Bloch, Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Research. ,vol. 20, pp. 1717- 1723 ,(1992) , 10.1093/NAR/20.7.1717
B. Smith, P. Selby, J. Southgate, K. Pittman, C. Bradley, G.E. Blair, Detection of melanoma cells in peripheral blood by means of reverse transcriptase and polymerase chain reaction. The Lancet. ,vol. 338, pp. 1227- 1229 ,(1991) , 10.1016/0140-6736(91)92100-G
A D van Mansfeld, J L Bos, PCR-based approaches for detection of mutated ras genes. Genome Research. ,vol. 1, pp. 211- 216 ,(1992) , 10.1101/GR.1.4.211
H. J. C. Rashleigh-Belcher, R. C. G. Russell, W. R. Lees, Cutaneous seeding of pancreatic carcinoma by fine-needle aspiration biopsy British Journal of Radiology. ,vol. 59, pp. 182- 183 ,(1986) , 10.1259/0007-1285-59-698-182
Hiroyuki Naito, Noboru Kuzumaki, Jun-ichi Uchino, Ryoji Kobayashi, Takaaki Shikano, Yorikazu Ishikawa, Shuzo Matsumoto, Detection of tyrosine hydroxylase mRNA and minimal neuroblastoma cells by the reverse transcription-polymerase chain reaction European Journal of Cancer and Clinical Oncology. ,vol. 27, pp. 762- 765 ,(1991) , 10.1016/0277-5379(91)90184-F
Tom Maniatis, Joseph Sambrook, E. F. Fritsch, Molecular Cloning: A Laboratory Manual ,(2001)
P.Sean Walsh, Joseph Varlaro, Rebecca Reynolds, A rapid chemiluminescent method for quantitation of human DNA. Nucleic Acids Research. ,vol. 20, pp. 5061- 5065 ,(1992) , 10.1093/NAR/20.19.5061
M. Almoguera, C., Shibata, D., Forrester, K., Martin, W.J., Arnheim, N. and Perucho, Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes Cell. ,vol. 53, pp. 549- 554 ,(1988) , 10.1016/0092-8674(88)90571-5