Protein domains governing interactions between E2F, the retinoblastoma gene product, and human papillomavirus type 16 E7 protein.

摘要: Human papillomaviruses (HPVs) are the etiological agents for genital warts and contribute to development of cervical cancer in humans. The HPV E7 gene product is expressed these diseases, genes from types 16 18 transformation mammalian cells. Mutation deletion analysis this suggests that transforming activity protein resides same domain as which directly involved complex formation with retinoblastoma (pRB). This one two conserved regions (designated CRI CRII) shared by other viral oncoproteins bind pRB, including adenovirus E1A protein. Binding type pRB has previously been shown affect pRB's ability DNA form complexes cellular proteins. In current study, we map functional interaction between monitoring association a 60-kDa version pRB60, transcription factor E2F. We observe CRII (amino acids 20 29), completely blocks binding full-length protein, necessary but not sufficient inhibit E2F/pRB60 formation. While 37 55) appears be compete E2F equivalent region neither nor sufficient. Only fragments contained both at least portion zinc-binding 60 98) inhibited These results suggest pRB60 associates through overlapping distinct domains.