Improving cost-efficiency of faecal genotyping: New tools for elephant species.

摘要: Despite the critical need for non-invasive tools to improve monitoring of wildlife populations, especially endangered and elusive species, faecal genetic sampling has not been adopted as regular practice, largely because associated technical challenges cost. Substantial work needs be undertaken refine sample collection preparation methods in order set quality provide cost-efficient that can effectively support management. In this study, we collected an extensive forest elephant (Loxodonta cyclotis) samples throughout Gabon, Central Africa, prepared them genotyping using 107 single-nucleotide polymorphism assays. We developed a new quantitative polymerase chain reaction (PCR) assay targeting 130-bp nuclear DNA fragment demonstrated its suitability degraded all three species. Using compare efficacy two recovery, found whole surface dung pile with swab stored small tube lysis buffer was convenient method producing high extraction success yield. modelled influence storage time on concentration recommendations optimized storage. The maximum ensure 75% months within 24 hours after defecation extended four one hour. Lastly, real-time PCR allowed us predict pre-screen samples, thus further increasing cost-efficiency our approach. recommend combining validation efficient method, build in-country capacity reduced development species-specific assays increase routine analyses expand use next-generation markers samples.