Preparation of EGFR monoclonal antibody conjugated nanoparticles and targeting to hepatocellular carcinoma
作者： Peifeng Liu , Zonghai Li , Mingjie Zhu , Ying Sun , Yaogang Li
DOI: 10.1007/S10856-009-3925-8
关键词: Hepatocellular carcinoma 、 Cancer cell 、 Flow cytometry 、 Cancer research 、 Monoclonal antibody 、 In vitro 、 Drug carrier 、 Epidermal growth factor receptor 、 Biochemistry 、 In vivo 、 Materials science
摘要: This study aims to determine the sensitivity, specificity and accuracy of epidermal growth factor receptor monoclonal antibody (EGFRmAb) modified poly(lactic acid-co-l-lysine) nanoparticles (PLA-PLL-EGFRmAb) NPs delivery system EGFR positive cancer cells. In study, a new PLA-PLL-EGFRmAb was prepared. The cellular cytotoxicity, uptake, targeted effect for hepatocellular carcinoma were investigated. vitro, findings Flow cytometry Confocal Laser scanning Biological Microscopy showed that can bind cells uptaken effectively. vivo in SMMC-7721 xenograft mouse model, could target tumor effectively, which demonstrated better targeting. These results have potential be used as carrier therapies.
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K. C. Podratz, N. J. Maihle, A. T. Baron, B. A. Barrette, C. H. Boardman, T. A. Christensen, E. M. Cora, J. M. Faupel-Badger, T. Greenwood, S. C. Juneja, J. M. Lafky, H. Lee, J. L. Reiter, EGF/ErbB Receptor Family in Ovarian Cancer Ovarian Cancer. ,vol. 107, pp. 247- 258 ,(2002) , 10.1007/978-1-4757-3587-1_11
Jiang Hua, Wang HuaMao, Gao HuiPing, Li ZongHai, Preparation and identification of a 9B9 monoclonal antibody specifically targeting EGFRvIII/EGFR. Chinese Journal of Cancer. ,vol. 16, pp. 391- 395 ,(2009)
Yourong Duan, Sen Xu, Qi Wang, Jie Liu, Zhirong Zhang, None, Optimization of preparation of DHAQ-loaded PEG-PLGA-PEG nonaparticles using central composite design Journal of Materials Science: Materials in Medicine. ,vol. 17, pp. 559- 563 ,(2006) , 10.1007/S10856-006-8940-4
Marion G. Anhorn, Sylvia Wagner, Jörg Kreuter, Klaus Langer, Hagen von Briesen, Specific targeting of HER2 overexpressing breast cancer cells with doxorubicin-loaded trastuzumab-modified human serum albumin nanoparticles. Bioconjugate Chemistry. ,vol. 19, pp. 2321- 2331 ,(2008) , 10.1021/BC8002452
Rajesh Singh, James W. Lillard, Nanoparticle-based targeted drug delivery Experimental and Molecular Pathology. ,vol. 86, pp. 215- 223 ,(2009) , 10.1016/J.YEXMP.2008.12.004
Omid Veiseh, Forrest M. Kievit, Jonathan W. Gunn, Buddy D. Ratner, Miqin Zhang, A ligand-mediated nanovector for targeted gene delivery and transfection in cancer cells. Biomaterials. ,vol. 30, pp. 649- 657 ,(2009) , 10.1016/J.BIOMATERIALS.2008.10.003
Giuseppe Di Lorenzo, Roberto Bianco, Giampaolo Tortora, Fortunato Ciardiello, Involvement of growth factor receptors of the epidermal growth factor receptor family in prostate cancer development and progression to androgen independence. Clinical Prostate Cancer. ,vol. 2, pp. 50- 57 ,(2003) , 10.3816/CGC.2003.N.013
Theresa M Allen, Pieter R Cullis, Drug Delivery Systems: Entering the Mainstream Science. ,vol. 303, pp. 1818- 1822 ,(2004) , 10.1126/SCIENCE.1095833
A-P. Meert, J-M. Verdebout, B. Martin, V. Ninane, F. Feoli, J-P. Sculier, Epidermal growth factor receptor expression in pre-invasive and early invasive bronchial lesions. European Respiratory Journal. ,vol. 21, pp. 611- 615 ,(2003) , 10.1183/09031936.03.00064902
Heidrun Wartlick, Kerstin Michaelis, Sabine Balthasar, Klaus Strebhardt, Jörg Kreuter, Klaus Langer, Highly specific HER2-mediated cellular uptake of antibody-modified nanoparticles in tumour cells. Journal of Drug Targeting. ,vol. 12, pp. 461- 471 ,(2004) , 10.1080/10611860400010697