Nannochloropsis spliced leader sequences and uses therefor
作者： Leah Soriaga , Rekha Seshadri , Robert C. Brown , Ariel S. Schwartz
DOI:
关键词: Biology 、 Gene 、 Recombinant DNA 、 Nucleic acid 、 Gene expression 、 Nannochloropsis 、 Genetics 、 Computational biology 、 Sequence (biology)
摘要: The present invention relates to the culture and manipulation of microorganisms for biotech applications, is based on discovery characterization spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, provides nucleic acid compositions comprising a SL sequence operably linked protein-encoding gene. Further provided are methods enhanced gene expression recombinant as well identification and/or isolation molecules tagged with sequence.
lens.org参考文章(24)
Yan Poon, Melisa Low, Shane Hopkins, Tanya Shin, Alvin Cho, Herbicide resistant organisms ,(2010)
Victor Klimyuk, Sylvestre Marillonnet, Stefan Werner, Yuri Gleba, Process of producing environmentally safe transgenic organisms ,(2002)
Ravi V. Durvasula, Subba R. Durvasula, Methods and compositions for control of disease in aquaculture ,(2007)
William John Michels, Molecular dissection method using trans-splicing to designed spliced leader sequences, genes, and constructs thereof ,(2004)
Cathy Chang, Eric J. Mathur, Nucleic acids and proteins and methods for making and using them ,(2006)
H. Zhang, Y. Hou, L. Miranda, D. A. Campbell, N. R. Sturm, T. Gaasterland, S. Lin, Spliced leader RNA trans-splicing in dinoflagellates. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 104, pp. 4618- 4623 ,(2007) , 10.1073/PNAS.0700258104
Huan Zhang, Senjie Lin, Retrieval of missing spliced leader in dinoflagellates. PLOS ONE. ,vol. 4, ,(2009) , 10.1371/JOURNAL.PONE.0004129
Mario Gustavo Mayer, Lucile Maria Floeter-Winter, Pre-mRNA trans-splicing: from kinetoplastids to mammals, an easy language for life diversity Memorias Do Instituto Oswaldo Cruz. ,vol. 100, pp. 501- 513 ,(2005) , 10.1590/S0074-02762005000500010
Huan Zhang, Yunyun Zhuang, Linda Hannick, Senjie Lin, Rita C. Kuo, Transcriptomic study reveals widespread spliced leader trans-splicing, short 5'-UTRs and potential complex carbon fixation mechanisms in the euglenoid Alga Eutreptiella sp. PLOS ONE. ,vol. 8, ,(2013) , 10.1371/JOURNAL.PONE.0060826
Hagar Zohar, Susan J. Muller, Labeling DNA for single-molecule experiments: methods of labeling internal specific sequences on double-stranded DNA Nanoscale. ,vol. 3, pp. 3027- 3039 ,(2011) , 10.1039/C1NR10280J