Granulocyte-Macrophage Progenitors as Candidate Leukemic Stem Cells in Blast-Crisis CML

摘要: methods We used fluorescence-activated cell sorting to isolate hematopoietic stem cells, common myeloid progenitors, granulocyte–macrophage and megakaryocyte–erythroid progenitors from marrow during several phases of CML normal marrow. BCR-ABL, b -catenin , LEF-1 transcripts were compared by means a quantitative reverse-transcriptase–polymerase-chain-reaction assay in cells progenitors. Confocal fluorescence microscopy lymphoid enhancer factor/T-cell factor reporter detect nuclear these cells. In vitro replating assays identify self-renewing as candidate leukemic the dependence self-renewal on activation was tested lentiviral transduction with axin, an inhibitor pathway. results The progenitor pool patients blast crisis imatinib-resistant expanded, expressed had elevated levels Unlike formed self-renewing, replatable colonies, capacity reduced enforced expression axin. conclusions Activation appears enhance activity potential