Sulfhydryl and disulfide groups of platelet membranes. I. Determination of sulfhydryl groups

摘要: Methods were evaluated for the quantitative measurement of free thiols in isolated platelet membranes. Two techniques found applicable utilizing either retentative capacity nitrocellulose filters 203Hg-labeled p-chloromercuri-benzoate adducts membrane proteins or spectrophotometric assay thiol thione anions released formation mixed disulfides between and disulfide reagents, 5,5′-dithiobis(2-nitrobenzoic acid) 6,6′-dithio-dinicotinic acid. Complete partial solubilization membranes with urea, sodium dodecyl sulfate phospholipase A resulted exposure additional to reaction 6,6′-dithiodinicotinic Approximately one half total was be masked native non-solubilized Kinetic studies on rates various SH reagents tested indicate presence at least two classes membrane. The reproducibility simplicity methods proved their general usefulness qualitative assays proteins.